Enzyme assays

Automation of enzyme assays.

The increasing use of enzymes as aids to clinical diagnosis has made it mandatory for clinical biochemists to develop techniques and instrumentation to enable large numbers of specimens to be processed quickly with adequate accuracy. The main objective in enzyme assay is to determine the initial reaction rate, as it is only during this period that there is a linear relationship between the rate...

Automated enzyme assays.

1 . Introduction 2. What is Automation? 3. Solution-Based, Absorption Spectrophotometric Methods 3.1. Specimen Identification 3.2. Specimen Preparation 3.3. Specimen Integrity 3.4. Pipeting Specimens 3.5. Temperature Control 3.6. Absorbance Accuracy 3.7. Wavelength Accuracy 3.8. Time Control 3.9. Cuvet Control 3.10. Control of Interferences 3.10.1. Keto Acids 3.10.2. Ammonium Ions 3.10.3. Glyce...

Enzyme assays in liver disease.

It is in the field of hepatology that enzyme assays are performed most frequently for diagnostic purposes. Their interpretation requires knowledge of many factors that influence the serum level of the particular enzymes assayed. Therefore, these factors are discussed briefly in relation to liver disease before considering important developments such as the value of estimating D-glutamyltransfer...

Enzyme assays in malignant disease.

Standardization of clinical enzyme assays.

During 1969, discussions between the Standards Subcommittee of the Laboratory Equipment and Methods Advisory Group of the Department of Health and Social Security and representatives of professional societies concerned with the assay of enzymes for diagnostic purposes led to the establishment of a working party to consider the current state of accuracy and reliability of diagnostic enzymology a...